In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.
- Group Size: For 8 Lab Groups
- Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended
- Time Required: Complete in 45 minutes
The kit includes:
- Instructions,
- Ready-to-Load QuickStrip™ DNA Samples
- UltraSpec-Agarose™
- Electrophoresis Buffer (50X)
- 10X Gel Loading Solution,
- FlashBlue™ DNA Stain
Additional required equipment & materials (not supplied with the kit):
- DNA Electrophoresis tank and accessories including casting trays and special power supply
- Micropipettes: 5-50 µl (Optional)
- Balance
- White Light Box
- Microwave or Hot Plate
*PLEASE NOTE: This kit has been developed for being suitable for use in education only and is not for diagnostic purposes*
